Oxidants and vascular smooth muscle.
نویسندگان
چکیده
( a ) 20 mM-sterol was co-sonicated with 20 mM-DOPC (1 mol/mol) and 0.2 mM12-doxy1 stearic acid. Spectra were measured at 3 7 T , pH 7.2, in a Brucker ER 200D e.s.r. spectrometer. ( b ) The (Ca2 +-Mg2+)-ATPase was purified and reconstituted in DMPC according to the methods of East & Lee [9]. Activities were measured using a coupled enzyme assay, at 3 7 T , pH 7.2, 2 mM-ATP, 1 pM-Ca2+ as outlined in East & Lee [9]. Sa-cholestan-3a-01, 0; 5a-cholestan-3P-ol, 0; SP-cholestan-3a-ol, 0; 5/3-cholestan-3P-ol, A ; and cholesterol reconstituted with DOPC-ATPase, V . BIOCHEMICAL SOCIETY TRANSACTIONS
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ورودعنوان ژورنال:
- Biochemical Society transactions
دوره 17 6 شماره
صفحات -
تاریخ انتشار 1989